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American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of chronic lung disease develops a severe pneumonia. Bronchoalveolar lavage fluid reveals the presence of numerous cysts containing multiple nuclei, which stain positively with silver stains but poorly with Gram stain. The patient's CD4+ T-cell count is within the normal range. The MOST likely causative organism and the preferred treatment are:

A) Blastomyces dermatitidis; itraconazole.
B) Paragonimus westermani; praziquantel.
C) Pneumocystis jirovecii; trimethoprim-sulfamethoxazole.
D) Histoplasma capsulatum; amphotericin B followed by itraconazole.

2. A microbiology laboratory is performing antimicrobial susceptibility testing using Etest strips. The MIC value is determined by:

A) The diameter of the zone of inhibition around the strip.
B) The presence or absence of growth at a single critical concentration.
C) The highest concentration that allows visible bacterial growth within the zone of inhibition.
D) The lowest concentration at which the elliptical zone of inhibition intersects the strip.

3. A clinical microbiology laboratory is preparing to validate a new molecular assay for the detection of methicillin-resistant Staphylococcus aureus (MRSA). To assess the positive predictive value (PPV) of the assay, they need to know:

A) The number of true positive results divided by the total number of positive test results.
B) The number of true positive results divided by the total number of true positive and false negative results.
C) The number of true negative results divided by the total number of negative test results.
D) The number of true negative results divided by the total number of true negative and false positive results.

4. A patient with HIV infection and a CD4+ T-cell count of 30 cells/µL develops progressive multifocal leukoencephalopathy (PML). The causative agent of PML is:

A) Epstein-Barr virus (EBV)
B) Cytomegalovirus (CMV)
C) Human herpesvirus 6 (HHV-6)
D) JC virus (JCV)

5. A patient develops a localized skin infection after being bitten by a tick. The infection is characterized by a slowly expanding erythematous rash with central clearing (erythema migrans). The MOST likely causative agent is:

A) Rickettsia rickettsii
B) Ehrlichia chaffeensis
C) Anaplasma phagocytophilum
D) Borrelia burgdorferi

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