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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. A normal hemoglobin molecule consists of a tetramer, of four heme and four globin chains, with a molecular weight of 64,500 daltons. Each of the four units can bind a molecule of oxygen for transport to the body's tissues. In the image shown below, there are four monomers (2 red globin chains and 2 blue globin chains) which form the entire hemoglobin tetramer structure. The green portions represent the 4 heme groups.
A normal hemoglobin molecule is comprised of the following:

A) Four heme and four globin chains
B) One heme and four globin chains
C) Four heme and one globin chains
D) Ferrous iron and four globin chains

2. Prozone effect (due to antibody excess) will result in an initial false negative in spite of the large amount of antibody in the serum, followed by a positive result as the specimen is diluted.
The prozone effect ( when performing a screening titer) is most likely to result in:

A) False negative
B) Mixed field reaction
C) False positive
D) No reaction at all

3. Protein in urine can be confirmed using sulfosalicylic acid (SSA) precipitation. The SSA reagent is added to a small volume of urine. Acidification causes precipitation of protein in the sample, which is subjectively graded as trace, 1+, 2+, 3+ or 4+. SSA reaction will detect albumin, globulins, and Bence-Jones proteins.
Which of the following would be the most appropriate method to confirm a positive protein from a urine dipstick:

A) Immunoelectropheresis
B) Heat precipitation
C) Protein electrophoresis
D) Sulfosalicylic acid precipitation

4. 2(2)2s = reject when 2 consecutive control measurements exceed the same mean plus 2s or the same mean minus 2s control limit.
Violation of this Westgard rule is usually caused by a systematic error.
Laboratory Operations
Which of the following describes the Westgard multirule 2(2)S?

A) One control data point falls outside +2s and a second point falls outside -2s
B) Two consecutive data points fall outside +2s
C) Two consecutive data points fall outside +2s or fall outside -2s
D) Two control data points are within + 2s
E) Synonymous with R4s

5. Which of the following CD markers is NOT present on a normal mature T cell?

A) CD7
B) CD3
C) CD5
D) CD20

Solutions: